S.T.A.R. Labs often provides scientific guidance for superheroes when they need it and have been instrumental in saving the world on many occasions. They have a strong influence in Metropolis and usually give assistance to the likes of Superman.
Over time, many have gotten aid from S.T.A.R Labs aside Superman, in the New 52 Static works at S.T.A.R. Labs New York and Cyborg was made there. The tech made at S.T.A.R. Labs is used in Red Robin's wings and kept Bart Allan together.
S.T.A.R. Labs (aka Swann Technology and Research) was first introduced into the "Smallville"-Continuity after the end of the TV Series. It was shown in the comics, that S.T.A.R. Labs was founded by Virgil Swann.
Arrow & The Flash
S.T.A.R. Labs first appeared the CW show Arrow, we see members Caitlin Snow and Cisco Ramone as research scientists. They later appear in the spin-off series The Flash where they play a major role in developing and learning about Barry Allen as he becomes the Flash.