A trade paperback collecting Y: The Last Man #24-31.
It's been nearly two years since Yorick Brown, the last man on Earth, set out to cross North America with his pet monkey Ampersand [the only other male mammal left alive after the plague of 2002 killed everything on the planet with a Y chromosome]. Now Yorick and his companions - government Agent 355 and bioengineer Dr. Allison Mann - have finally reached Dr. Mann's laboratory in San Francisco, where she can begin trying to unravel the secret of Yorick and Ampersand's survival.
After months of constant travel, staying in one place is a blessed relief - and also a chance for the various pursuers stalking the trio to finally catch up with them. But while Yorick's band can confront these newly arriving threats head-on, it's the danger they cannot see which may mean the end for the last man - and with him any hope for mankind's future.
This volume collects three stories: "Tongues of Flame" (#24-25), "Hero's Journey" (#26), "Ring of Truth" (#27-31).