Tsunade appears in 366 issues.
Danzo is the leader of Root and operates in the shadow of Konoha, planning to complete his goals. When he was young, he trained under the Second Hokage Tobirama with the would-be Third Hokage Hiruzen, who he shared a close friendship with. As Tsunade was recovering from the attack on Konoha by Akatsuki leader Pain, Danzo was named interim Sixth Hokage in her stead.
Tsunade has sent out an order to capture all Akatsuki members, including Deidara.
Hanzo was the leader of the Rain village who defeated Jiraiya, Orochimaru, and Tsunade in their youth. He is the one that titled them the "Sannin."
Itachi Uchiha is the older brother of Sasuke Uchiha. He was of the Uchiha Clan of Konohagakure and eventually became a Konoha missing-nin. He was a member of the Akatsuki. He was the best friend of Shisui Uchiha. He was considered a prodigy by the Uchiha due to his talents and intelligence
Kabuto is one of Orochimaru's most loyal followers, and he also has fought Tsunade on one occasion, nearly defeating her were it not for the help of Naruto and Shizune.
Kaguya Ōtsutsuki was a princess and Matriarch of the Ootsutsuki clan from the era long before the formation of the ninja clans, who ate the forbidden fruit from the Divine Tree and was the first person to use Chakra.
Kakuzu is a greedy bounty hunter. He does not like having a partner because he is prone to violent outbursts in battle that have claimed the lives of his partners, however his new partner is special.
Kisame was originally from the Hidden Mist village. He was part of the Seven Swordsman along with Zabuza and is Itachi's partner in Akatsuki.
Kurama, more commonly known as the Nine-Tails, is one of the nine tailed beasts created by the Sage of the Six Paths. For centuries, it has always been feared but sought after to be used as a tool for war by countless humans. During its attack on Konohagakure, the Fourth Hokage sacrificed his life to seal the fox into his infant son, Naruto Uzumaki.
Madara Uchiha was once the leader of the famed Uchiha Clan who aided in the founding of Konohagakure no Satō (Hidden Leaf Village). He battled with the First Hokage, Hashirama Senju, over the issue of leadership. It is then revealed that he is the one who influenced Tobi/Obito to carry out Project Tsuki no me (Eye of the Moon Plan). Madara was the first uchiha ever to awaken the Mangekyo Sharingan.
Tsunade fought against Manda in the sannin battle. She was the first one to both truly hurt and beat Manda.
Used to be on Orochimaru's team. Have been enemies ever since e betrayed Konoha.
The mysterious and powerful leader of Akatsuki, a band of S-rank missing-nin. He possesses the Sage's revered Rinnegan, the eyes of reincarnation. A student of the Toad Sage, Jiraiya, and a descendant of the Uzumaki clan.
Tobi, born under the name of Obito Uchiha, is the true true leader of the Akatsuki. He was part of a Genin team with Kakashi and Rin and trained by the Fourth Hokage, Minato. Thought dead and after witnessing the death of his beloved Rin, he was saved by Madara Uchiha and chose to turn his back on his village and take over the world. He was the Jinchūriki of the 10 tails.
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