Super Soldier Serum appears in 158 issues.
Doctor Cornelius for the most part was blackmailed into joining the Weapon X Program. He was a fugitive on the run from the United States known as the "Mercy Killer" quack.
The creator of the Super Soldier Serum that created Captain America. He was assassinated before writing down the formula, which led to several parties attempting to recreate the formula from scratch with little success.
First became the Destroyer, then later followed his father's mantle as Union Jack the second. He was the brother of Spitfire, whom also became part of the team of Invaders
During World War II, Steve Rogers volunteered to receive the experimental Super-Soldier Serum. Enhanced to the pinnacle of human physical potential and armed with an unbreakable shield, he became Captain America. After a failed mission left him encased in ice for decades, he was found and revived by the Avengers. Later joining their ranks and eventually becoming the team's leader.
Roger Aubrey was a spy who worked behind enemy lines during World War Two, first known as the size-changing Dyna-Mite and later as the Destroyer - an identity he adopted from his boyfriend Brian Falsworth.
A former member of the Serpent Society. After she fell in love with Captain America, she was inspired to switch sides and dedicate herself towards the cause of good.
The head scientist of Weapon Minus program who injected himself with a mix of Super Soldier serum and LSD.
Steven Rogers [formely William Burnside] was the fourth Captain America and was active in the 1950s. He has resurfaced since often fronting extremist causes and appearing as an antagonist to the real Captain America.
Isaiah Bradley is the last surviving test subject of Dr. Reinstein's portion of Project: Rebirth to which he was an unwitting participant.
A former sidekick to Captain America, Jack Monroe abandoned his mentor when he became disillusioned with the hero's lack of finite action against the super-villains they fought. He later became the notorious serial killer known as Scourge.
Jake Niman was an Afghanistan war vet who was caught in a crossfire that injured him and killed his family. A Super Soldier Serum variant was used to transform him into Johnny Nightmare.
The son of Isaiah Bradley, with all the abilities that the Super Soldier Serum grants. He joined James Rhodes Crew to defend against crime in the inner city.
Man-Thing is a large semi-intelligent creature that dwells in the Florida Everglades. He is the sole guardian of the Nexus of All Realities located there.
Master Man was a frail Nazi powered by the Super Soldier Serum who fought Captain America in World War II.
Barbara "Bobbi" Morse is an agent of S.H.I.E.L.D. and has served on several Avengers teams.
A founding member and leader of the Young Avengers, Elijah Bradley is the nephew of Josiah-X and grandson of Isaiah Bradley. After receiving a blood transfusion from his grandfather, Elijah now possesses the abilities of a Super Soldier. Patriot recently resigned his title of Patriot and now lives with his mother.
A candidate for Project: Rebirth, Clinton McIntyre was given an incomplete version of the super-soldier serum but after proving unstable, he was cryogenically frozen. Half a century later he was discovered by the terrorist group A.I.M. who manipulated him into being their agent.
A long-time enemy of Captain America, Johann Schmidt is a proud Nazi general and the embodiment of evil, fear, and horror. He is known as the ruthless Red Skull.
After ingesting an experimental super-soldier formula, the lowly Robert Reynolds became the Sentry, one of the first and mightiest superheroes. He was trapped in a constant battle with his dark side known as the Void. After his membership with the Avengers, he joined Norman Osborn's Dark Avengers and died after single-handedly destroying Asgard. More recently, the Sentry was resurrected by the Apocalypse Twins as one of their Horsemen, the Horseman of Death.
Tony Masters (a.k.a. Taskmaster) possesses the ability to absorb knowledge and instantly replicate the combat skills of anyone he observes. He has used these skills as a teacher to many super villains, henchmen, and even some heroes. In order to clean his record, he started working for the U.S. government as a combat instructor. Due to a set of events, he was ultimately put in as sheriff of the criminal island known as Bagalia. He currently serves as Madame Hydra's personal bodyguard.
Julia Koenig was Nazi spy Madame Rätsel (a.k.a. Madame Mystery) during World War II.
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