The Splinter Clan arises after the death of Splinter in the events that occurred in Teenage Mutant Ninja Turtles #100. After this situation the Teenage Mutant Ninja Turtles, after a period of duel, decide to return to the Mutant Town to help all the people who were converted. on mutants after Old Hob's mutagen bomb. To this end, they decide to settle in a building and create a martial arts school where they can teach self defense, calligraphy and other issues that allow the people of the city to survive. To do this, Leonardo, Michelangelo, Donatello, Raphael and Jennika decide to call themselves the Splinter Clan, and become protectors and promoters of the new mutant city. A short time later, Alopex joins them as a main part of the ninja clan by decision of all the turtles and a full member of the family. Furthermore, the Splinter Clan has also become home to several orphan mutants.