Peter Parker was bitten by a radioactive spider as a teenager, granting him spider-like powers. After the death of his Uncle Ben, Peter learned that "with great power, comes great responsibility." Swearing to always protect the innocent from harm, Peter Parker became Spider-Man.
@p00p122: I know one version does have an X-Gene that activated later in life after his spider-powers, found him on a wiki trawl a while back, earth-13270 pretty sure.
Not to my knowledge, but given how many times Spider-Man has been mistaken for a Mutant, you'd think at least one writer would have gone with the twist of him being a Mutant for real. But, to my knowledge, it's never happened. I could be wrong though.
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