Peter Parker was bitten by a radioactive spider as a teenager, granting him spider-like powers. After the death of his Uncle Ben, Peter learned that "with great power, comes great responsibility." Swearing to always protect the innocent from harm, Peter Parker became Spider-Man.
The first true love of Peter Parker. She's best known for her tragic death by being thrown off from the Brooklyn Bridge by the Green Goblin. The Earth-65 version is Spider-Gwen who is a major player in the Spider family.
J. Jonah Jameson is the former employer of Peter B. Parker and remains an aggravate of Spider-Man. He was once the chief editor and publisher of the Daily Bugle, a New York newspaper, and the mayor of New York before his resignation amidst scandal resulting from Superior Spider Man and the Goblin Nation. He now is a host of a show on the F.A.C.T. News Channel
The second most powerful member of the Fantastic Four and the younger brother of Sue Richards. Due to an accident caused by cosmic radiation in space, he can manipulate fire, turn his entire body into it and fly. While Johnny is known for his impetuous and sometimes reckless nature, he is also a loyal friend and fearless hero.
Phil Urich, was a hero who, somewhere along the line, began thinking the universe was against him, leading to a psychotic break. Formerly taking up the mantles of Darkhawk, Green Goblin and Hobgoblin, Urich is now known as the Goblin Knight.
Hank Pym is a scientific genius, a founding member of the Avengers, the creator of Pym Particles and of Ultron, and a modern-day superhero who suffers from a Bipolar disorder. He has acted under many memorable identities such as Ant-Man, Giant Man, Goliath, Yellowjacket, and the Wasp. He is also the Earth's Scientist Supreme, as decreed by Eternity.
Harry Osborn is the best friend of Peter Parker, the Amazing Spider-Man. His father is Norman Osborn, a global industrialist and billionaire.
A former investigative journalist for the Daily Bugle and current owner of the newspaper Front Line who uses his friendships with Daredevil and Spider-Man to get information on criminals.
Roderick Kingsley became a worthy heir of the Goblin legacy, creating his own identity, of the Hobgoblin, rather than being just another Green Goblin clone.
Normie Osborn is the son of Harry Osborn and Liz Allan Osborn.
Molten Man is the stepbrother of Liz Allen. He has had several run-ins with Spider-Man. He was recently cured of his condition.
Mary Jane Watson is a major love interest of Peter Parker aka Spider-Man. She married him but didn't(!), now she's one of the few people who know who he is, and still supports him as a close friend and confidante. Currently, Mary Jane and Peter have reconciled their relationship and she no longer seems to work for Stark Industries.
The second Hobgoblin, Jason Macendale, became possessed by a demon after making a deal with N'astirh for power. When Jason rid himself of the demon he was host to, it became a seperate entity known as Demogoblin.
Elizabeth Allan was a classmate of Peter Parker. She dated and later married Harry Osborn, Peter's best friend. She has a son named Normie Osborn.
As a child, Matt Murdock was blinded by radioactive waste while trying to save an elderly stranger about to get hit by a truck carrying the dangerous material. In turn, his other senses were heightened to superhuman sharpness and he gained a form of "radar sense". By day, he is a successful trial lawyer; but by night, he guards Hell's Kitchen as Daredevil: the Man Without Fear.
Mendel Stromm is a chemist and robot expert. He was Norman Osborn's business and scientific partner until Norman betrayed him. His consciousness operates out of a robotic body.
The amoral industrialist Norman Osborn was transformed by an experimental serum into the maniacal Green Goblin, and has endured over the decades as Spider-Man's most dreaded archenemy. Osborn is known for being the killer of Gwen Stacy, the architect of the Clone Saga, and the one-time director of S.H.I.E.L.D.
Betty Brant's older brother who got into gambling debt with a gangster named Blackie, and the third person to become the Crime-Master.
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