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Peter Parker was bitten by a radioactive spider as a teenager, granting him spider-like powers. After the death of his Uncle Ben, Peter learned that "with great power, comes great responsibility." Swearing to always protect the innocent from harm, Peter Parker became Spider-Man.
Harry Osborn is the best friend of Peter Parker, the Amazing Spider-Man. His father is Norman Osborn, a global industrialist and billionaire.
Edward Whelan was transformed by the second Baron Zemo into the hideous, beastly, rat creature that he is today.
As a child, Matt Murdock was blinded by radioactive waste while trying to save an elderly stranger about to get hit by a truck carrying the dangerous material. In turn, his other senses were heightened to superhuman sharpness and he gained a form of "radar sense". By day, he is a successful trial lawyer; but by night, he guards Hell's Kitchen as Daredevil: the Man Without Fear.
Former New York City Police Department officer and Peter Parker's ex-girlfriend. She was briefly transformed by the Goblin Serum, but has since been returned to normal.
A man who would later take the name of Martin Li, was given an experimental drug which eventually allowed him control of some type of black electrical energy resembling a photographic negative.
Harry Osborn's fiance who absorbed a unique variation of the Goblin Serum that allows her to shapeshift into a goblin. After losing those powers, she assumed the persona of Queen Cat.
Rumor monger and scandal sheet printer, Dexter Bennett became the latest owner of the Daily Bugle after a hostile buyout of long-time owner J. Jonah Jameson.
A police officer in New York. Doesn't have faith that Spider-Man is a good guy.
A villain who uses nanites to alter motor vehicles to his liking. Formerly working for Mr. Negative, he is now a member of the Sinister Six.
A super-powered bank robber. He has the power to break things by touching them. He's has been beaten by Spider-Man.
13 year old daughter of Kraven the Hunter. Now walking in her father's footsteps, she continues the hunt for Spider-Man.
Sasha was married to Kraven the Hunter.
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