Spider-Man 3 released on May 04, 2007.
Peter Parker's aunt-by-marriage and one of the most important people in his life.
Betty Brant started as a personal secretary at the Daily Bugle and later became a top reporter. Betty is also one of the many love interests of Peter Parker and ex-girlfriend of Flash Thompson
Eddie Brock bonded to the alien symbiote after it was discarded by Spider-Man, becoming the first Venom. Brock was later Anti-Venom, but the symbiote was lost during Spider-Island. Brock has also been forcibly bonded to the Toxin symbiote by the Crime Master, but has reluctantly given it up to stop Carnage. He has recently been rebonded with Venom.
Flash attended the same high school and university as Peter Parker, who would later be known as Spider-Man. He frequently bullied Peter but was actually Spider-Man's greatest fan. In time Flash befriended Peter, becoming a decorated war hero and was bonded to the Venom symbiote. Some time after losing the symbiote, Flash became the new Anti-Venom.
A retired New York Police Department captain and the father of Gwen Stacy.
The first true love of Peter Parker. She's best known for her tragic death by being thrown off from the Brooklyn Bridge by the Green Goblin. The Earth-65 version is Spider-Gwen who is a major player in the Spider family.
Harry Osborn is the best friend of Peter Parker, the Amazing Spider-Man. His father is Norman Osborn, a global industrialist and billionaire.
J. Jonah Jameson is the former employer of Peter B. Parker and remains an aggravate of Spider-Man. He was once the chief editor and publisher of the Daily Bugle, a New York newspaper, and the mayor of New York before his resignation amidst scandal resulting from Superior Spider Man and the Goblin Nation. He now is a host of a show on the F.A.C.T. News Channel
Keemia Alvarado is the daughter of Alma Alvarado and the Sandman. When Sandman found out about Keemia, he tried to be a part of her life but his involvement ended up putting her into a foster home.
During an experiment Dr. Curtis Connors accidentally turned into a ferocious reptilian monster who seeks to turn humans into lizards like himself. The Lizard is one of Spider-Man’s oldest and deadliest foes while ironically, his alter ego is a close friend and former teacher of Spidey’s alter ego Peter Parker.
Mary Jane Watson is a major love interest of Peter Parker aka Spider-Man. She married him but didn't(!), now she's one of the few people who know who he is, and still supports him as a close friend and confidante. Currently, Mary Jane and Peter have reconciled their relationship and she no longer seems to work for Stark Industries.
The amoral industrialist Norman Osborn was transformed by an experimental serum into the maniacal Green Goblin, and has endured over the decades as Spider-Man's most dreaded archenemy. Osborn is known for being the killer of Gwen Stacy, the architect of the Clone Saga, and the one-time director of S.H.I.E.L.D.
Editor-in-Chief of the Daily Bugle, and perhaps the best friend to J. Jonah Jameson.
Former enemy of Spider-Man and the Fantastic Four, Sandman reformed after realising he was unhappy with his life. He joined the Avengers and later Silver Sable's Wild Pack. Sandman eventually returned to crime after being brainwashed by the Wizard, but sometimes still displays desire to better himself.
The third Spider-Man movie. It features the venom symbiote.
A burglar Spider-Man allowed to escape at one point, he would later kill Uncle Ben.
Peter Parker's uncle and the father figure whose death was the main inspiration for Peter becoming Spider-Man. Ben Parker taught Peter that with great power comes great responsibility.
The Venom symbiote bonded with Spider-Man during his adventure in the first "Secret Wars" on the Beyonder's makeshift planet called Battleworld. Initially, he thought the symbiote was just a costume. However upon his discovery that it was sentient, he rejected it. Since then, the symbiote has bonded with several different hosts. It's current host is Eddie Brock.
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