When Spider-Man came to the astral plane, Shathra became aware of his presence and soon came to the real world to feed the Spider to her offspring. She took a human form and came to Fox News to proclaimed herself to be the lover of Spider-Man to disgrace him. Spider-Man showed up and angrily punched Shathra, then she revealed her true form. They fought and Shathra paralyzed Spider-Man with her venom. He was later saved by Ezekiel.
They traveled to Africa, to lure Shathra to the Spider Temple. There, they fought her until she was swarmed by Spiders, and then eaten.
Shathra assumes the form of Annabelle Adams in order to seduce Kaine and the two sleep together. Shathra after her previous fight with Spider-Man was looking for a suitable father for her children.
Upon Kaine's rejection she attempts to kill him and everyone around him but is thwarted when Kaine turns into his "Other" form and beats her down. It is unknown if Shathra is dead or alive after her confrontation with Kaine.
She has the totem of a Spider-Wasp, so she is driven by instinct to eat the totem of a Spider (namely Peter Parker). Shathra seems much stronger than Spider-Man, almost to that level of Morlun. She is also very agile. She can shoot stingers from her wrists, that are coated with paralyzing venom.
During her fight with the Scarlet Spider she demonstrated incredible healing by taking one of Kaine's stingers through her head and healing instantly. She also showed the ability to transform into a swarm of wasps.