Seward Trainer appears in 55 issues.
A geneticist from the 1930s who became obsessed with further evolution experiments which he used on himself. Depending on what he hopes to gain from whatever his current experiments are, he has been a force for the greater good, and for his own dark purposes.
The Jackal is an enemy of Spider-Man who played a big role in both of the Clone Sagas. He has since seemingly returned to infest Manhattan with spider-powers for his own sinister ends, organizing test subjects into a spider-powered army.
Otto Octavius' apprentice, who took the title of her mentor after Octavius' death, and has fought Spider-Man many times.
The amoral industrialist Norman Osborn was transformed by an experimental serum into the maniacal Green Goblin, and has endured over the decades as Spider-Man's most dreaded archenemy. Osborn is known for being the killer of Gwen Stacy, the architect of the Clone Saga, and the one-time director of S.H.I.E.L.D.
Mendel Stromm is a chemist and robot expert. He was Norman Osborn's business and scientific partner until Norman betrayed him. His consciousness operates out of a robotic body.
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