Sam Alexander appears in 496 issues.
Aaron Davis is the uncle of Miles Morales and originally served as the Ultimate Prowler. After dying in an accident during a fight with his nephew, Aaron was resurrected years later after Secret Wars. He's now assumed the identity of the Iron Spider, and formed a new Sinister Six.
Annihilus, a native of the planet Arthos, is the ruler of the Negative Zone and longtime foe of the Fantastic Four. He is a nihilist who is obsessed with finding different ways to extend his lifespan and isn't opposed to destroying all that which he sees as a threat to his existence.
Recruited out of high school by the Maggia to counter S.H.I.E.L.D's hero program. He was trained to know Spider-Man's every move.
A member of the group of Spaceknights called the Disavowed of Galadoria.
The Blood Brothers were two linked alien brothers who served as minions of Thanos.
Carnage is the offspring of the alien symbiote Venom. It bonded with the crazed serial killer, Cletus Kasady, thus creating one of Spider-Man's most murderous, brutal, and lethal adversaries.
Celestial who attacked Earth.
The first X-Man, Scott Summers possesses the mutant ability to draw ambient energy from another dimension through his eyes, which are actually portals to said dimension. Usually channeling this energy into concussive force blasts, he is visually distinctive for the ruby quartz visor he wears to control his devastating power. A born leader, Cyclops succeeded his mentor Professor X to command the X-Men.
Member of the Slaughter Squad.
Recruited by Maggia out of high school to counter S.H.I.E.L.D's hero program. Trained to battle Sam Alexander.
Diamond skinned crook and one of Nova's greatest enemies.
Dr. Midas was a scientist who craved power and wanted to genetically alter his body in the same way as the Fantastic Four.
A former nuclear physicist and atomic researcher, Otto Octavius' life changed forever when one of his experiments went horribly wrong. From that day forward, he has set out to prove his superior intellect in a number of ways, but these plans are usually thwarted by Spider-Man.
Daughter/prisoner of Doctor Midas. Exterminatrix became an ally to Noh-Varr, the newly-proclaimed Marvel Boy.
Fang is an alien member of the Shi'ar Imperal Guard. He is mostly known for having his costume taken by Wolverine, whom used it for some time.
A small-time thug who battled Spider-Man on several occasions, the Fly was killed by the Scourge of the Underworld. He was later brought back by The Hood, and received power enhancements. Now Fly is member of Crime-Master's Savage Six.
Senator Patrick drafted the Underage Superhuman Welfare Act, a law that outlawed superheroes below twenty-one years of age.
A geneticist from the 1930s who became obsessed with further evolution experiments which he used on himself. Depending on what he hopes to gain from whatever his current experiments are, he has been a force for the greater good, and for his own dark purposes.
Proxima Midnight's slave sent by Thanos to hunt down Sam Alexander.
Sam Alexander's schoolmate who constantly bullies him.
Kluh who was originally intended to be the genetically altered and "improved" clone of Hulk created by Xemnu the Living Titan. However in current issues of the AXIS event Kluh is referred as the inverted version of Hulk, which contrary to Hulk's rage-based powers, this Hulk version becomes what it is by sadness and depression.
The Leviathon Mother was a giant and monstrous entity and the matriarch of the Leviathon Tide, who launched an invasion of Earth.
The one time ruler of Latveria during Secret War and a cyborg
The Master is a super-villain and an arch-nemesis of Alpha Flight.
son of the Mole Man
A successor to the original Orb, this one has an actual giant eyeball for a head.
Recruited out of high school by the Maggia to counter S.H.I.E.L.D's hero program.
A demented opera clown that leads a gang of mysterious individuals garbed in classical opera costumes.
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