A previous character by the same codename, nicknamed Queenie, appeared only in Justice League of America #43 (March, 1966), #54 (June, 1967) and Joker #5 (January, 1976). In the three part storyline of Justice League of America #203-205, as the Queen of Spades, she disguised herself as Lois Lane and Sue Dibny by the power of the Sceptre Royale in order to trick both Superman and Elongated Man. She reappeared in JLA Classified #22 (July, 2006) only to have a retconned death, killed in a rock fall.
Queen appeared alongside the rest of the Royal Flush Gang in the episode "The Wild Cards".
Queen appeared in several episodes as a member of the futuristic Royal Flush Gang. In this version she was the mother of Ten and Jack. She was voiced by Amanda Donohoe and later Sarah Douglas.
A teenage version of Queen appears in the episode "Wild Cards" as a member of the first Royal Flush Gang assembled by the Joker and Harley Quinn. Queen was apparently killed after being caught in an explosion caused by the Joker, but later appeared alive and well in the cartoon's spin-off comic series. She was voiced by Tara Strong.
Another version of Queen briefly appeared in the episode "Epilogue". This version of Queen was an overweight transvestite.
A past iteration of Queen appears in the episode "Return of the Fearsome Fangs!" as a member of a Royal Flush Gang chapter from the 1800's. Like the other gang members, this version of Queen was styled to look like an Old West outlaw.
The Queen appeared in the episode "Legacies" and was played by Sarah Jane Redmond as a member of a more realistic and grounded version of the Royal Flush Gang.
Queen appears as a member of the Royal Flush Gang chapter faced by the Justice League at the start of the film. She is quickly defeated and tied up by Wonder Woman. She was voiced by Grey DeLisle.