Honorable Mentions;
Hammerhead, Silvermane, Man-Wolf, Hydro-man, Beetle, Molten Man, Spider-
Slayer, Tombstone, Jackal.
Note; J Jonah Jameson is technically more of an antagonist than a villain and lately Kaine is more of an anti-hero.
Director of H.A.M.M.E.R., Gwen Stacey's killer, CEO of Oscorp, the leader of the Dark Avengers, Spiderman's insane arch-enemy, has gone from being the Green Goblin to the Iron Patriot.
The founder of the Sinister 6 and the master planner, he recently took Spiderman's body and life and has become the brutal, Superior Spiderman.
The greatest Venom has been Eddie Brock who (though being Spiderman's arch-enemy) wouldn't hurt innocents, Mac Gargan was a monster and the latest Venom, Flash Thompson is a government agent.
Kraven is one of the great Spiderman villains, why? He almost killed Spiderman in Kraven's Last Hunt in which he shot himself at the climax, believing he'd won.
Dr Curt Connors is an ally of Spiderman's but his alter ego definitely isn't, the Lizard has basically taken over completely now even eating his wife and son.
J. Jonah Jameson has been a critic and antagonist of Spiderman's pretty much ever since Spiderman's first appearance, not knowing one of his employees was in fact the web-head.
Vulture's another of the oldest villains and a member of the original Sinister Six.
Mysterio isn't the greatest of villains nor one of the most powerful but he's an underdog villain who's pretty cool.
Roderick Kingsley is the greatest Hobgoblin so far and one of the most interesting villains, tricking readers into thinking that Flash Thompson was the Hobgoblin.
Electro is another villain who has plagued Spiderman for the majority of his career.
Shocker's a pretty basic and insignificant villain but his gloves are awesome and you have to give the guy props for never giving up his vendetta against Spiderman.
Sandman is one of the oldest foes of Spiderman and an occasional ally, his powers are pretty awesome and he was one of the original Sinister Six.
Scorpion was one of the main villains in Marvel Knights: Spiderman and one of the few who knew his secret identity, before becoming Venom he was a pretty awesome villain.
Rhino almost killed Madame Web but apart from that he's a pretty insignificant villain.
He was the first Spiderman villain and is the half-brother of Kraven the Hunter.
Kaine is one of the earliest clones of Spiderman and was more of a villain to Ben Reilly but since he's part of the Spiderman Universe I put him on the list.
Carnage is the spawn of Venom and ruthless killing machine without humanity, this guy is so powerful it took Spiderman and Venom to take him down.
He killed Spiderman ('nuff said).
He cloned Spiderman and is the mastermind behind The Clone Saga and Spider-Island.
The man who shot and killed Uncle Ben has plagued Spiderman since his first issue, he isn't exactly a great villain but his significance can't be overstated.
