Heroes of the Spiderverse
Heroes with a spider-theme:

a.k.a. Peter Parker; Original Spider-Man
a.k.a. Miguel O'Hara
a.k.a. Jamoff "Max" Borne
a.k.a. Spider-Man II
a.k.a. Jessica Drew
a.k.a. Spider-Woman II
a.k.a. Spider-Woman III
Spider-Woman of Earth-1610. She isn't a real girl but a female clone of Peter Parker - Spider-Man.
a.k.a. Anya Corazon
a.k.a. Spider-Kid; a.k.a. Ollie Osnick.
Japanese Spider-Man
Spider-Man III (MC2)
a.k.a. Peter Porker
The daughter of Peter Porker, the Spider-Ham, May Porker continues her father's legacy as Swiney-Girl.
a.k.a. Piguel O'Hara
Combination of Spider-Man & Superboy
Combination of Spider-Man 2099 & Superboy
a.k.a. Scarlet Spider II (MC2)
Psychic friend of Spider-Man
Ultimate Spider-Girl from earth- 1610.
Spider-Man of the 1940-1950's
Friend of Spider-Man
a.k.a. Venom
a.k.a. Anti-Venom II
a.k.a. Tobey Maguire
A team of heroes who wear the Iron Spider costume; they work for The Initiative.
One of the Scarlet Spiders team. Last surviving member of that team.
One of the Scarlet Spiders team.
One of the Scarlet Spiders team.
Bears the name of one of the most deadly types of spider.
Granddaughter of Spider-Man in the Old Man Logan Universe. Goes by Spider-Girl now.
Formerly Deformed clone of Peter Parker. Now the hero known as Scarlet Spider.
Enhanced clone of Peter Parker in the Ultimate Universe.
Spider-Man III
is he a hero? who knows.
Earth-65 Gwen Stacy is heroine known as Ghost Spider.
a.k.a. Cindy Moon
Spider-Man: India
a.k.a. Peni Parker
a.k.a. Anna May Parker
a.k.a. The Superior Spider-Man
Daughter of Spider-Man and Mary Jane from alternate reality.
a.k.a. Spider-Ma'am; from an alternate reality.
a.k.a. Spider-Ben; in several alternate realities.
a.k.a. Spider-girl; from an alternate reality.
a.k.a. Captain Spider; from an alternate reality.
Daughter of Spider-Man and Mary Jane from alternate reality(MC2). Goes by Spider-Woman now, in honor of her late father.
Spider-Woman in a variety of different universes.
I don't know much about her.