Mysterio (Berkhart) appears in 46 issues.
Peter Parker's aunt-by-marriage and one of the most important people in his life.
The second and most successful clone of Spider-Man created by the Jackal. A superhero known as the Scarlet Spider, Reilly later replaced Peter Parker for a time as Spider-Man after he found out that all this time he was the original and not Peter. Ben met his ultimate demise in the fight against the Green Goblin which led to Peter becoming Spider-man once again, after he found out that he is the real Spider-man after all.
As a child, Matt Murdock was blinded by radioactive waste while trying to save an elderly stranger about to get hit by a truck carrying the dangerous material. In turn, his other senses were heightened to superhuman sharpness and he gained a form of "radar sense". By day, he is a successful trial lawyer; but by night, he guards Hell's Kitchen as Daredevil: the Man Without Fear.
A former nuclear physicist and atomic researcher, Otto Octavius' life changed forever when one of his experiments went horribly wrong. From that day forward, he has set out to prove his superior intellect in a number of ways, but these plans are usually thwarted by Spider-Man.
Flash attended the same high school and university as Peter Parker, who would later be known as Spider-Man. He frequently bullied Peter but was actually Spider-Man's greatest fan. In time Flash befriended Peter, becoming a decorated war hero and was bonded to the Venom symbiote. Some time after losing the symbiote, Flash became the new Anti-Venom.
Francis Klum is a teleporting mutant who acquired the Mysterio equipment to seek revenge against Spider-Man.
J. Jonah Jameson is the former employer of Peter B. Parker and remains an aggravate of Spider-Man. He was once the chief editor and publisher of the Daily Bugle, a New York newspaper, and the mayor of New York before his resignation amidst scandal resulting from Superior Spider Man and the Goblin Nation. He now is a host of a show on the F.A.C.T. News Channel
A sadistic mercenary abducted as a kid by Crime-Master who raised him to be a stone-cold killer. He assumed the persona of Jack O'Lantern after killing all of the people who previously used this name.
A former boxer whom is friends with the Amazing Spider-man
Mary Jane Watson is a major love interest of Peter Parker aka Spider-Man. She married him but didn't(!), now she's one of the few people who know who he is, and still supports him as a close friend and confidante. Currently, Mary Jane and Peter have reconciled their relationship and she no longer seems to work for Stark Industries.
Editor-in-Chief of the Daily Bugle, and perhaps the best friend to J. Jonah Jameson.
The Principal of Midtown High School who hired Peter Parker to be the new Science teacher and Flash Thompson as the School Coach. Principal Harrington was tragically killed by the Chameleon.
Stewart Ward is a human/Z'nox hybrid with connections to HYDRA.
X-Man is an Omega-level mutant genetically created by Mr. Sinister from the Age of Apocalypse timeline. He shares the same genetic history as Cable. After returning from the dead, Nate joined the New Mutants and started a relationship with Dani Moonstar, until his full powers returned.
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