Hunter makes his appearance in "Supernatural: Origins" issue #2. He follows John Winchester to the cemetery in Eureka, California where John and Jacob were searching for a Black Shuck. Hunter watches as Jacob is killed and then saves John. He takes John under his wing and becomes John's trainer in the art of demon hunting.
Hunter is known to the crowd at Harvelle's Roadhouse where the supernatural is a way of life. It's a gathering spot for the hunters to rest and share information.
He is responsible for taking John on his second hunt which leads to John's first kill, a shapeshifting demon.