Peter's webshooters, the soles of his boots, and the soles of Mile's booths, in addition to the art style, confirm this poster is related to the upcoming 2017 cartoon.
I liked the addition of Miles and Gwen because they've got cool costumes and origins, and they fit the mythos well. Anya--not so much. This seems to make the Spider Island rumors more credible, so I think Anya will likely have an altered origin. Can't get behind her hair and lower face showing though. And 4 spiders seems to be my breaking point. I know they want to sell merchandise, but c'mon now.
Speaking of merchandise, Anya doesn't appear on as much as the others. Hopefully that means she's not sticking around as long...
On the bright side, I'm looking forward to purchasing Peter, Miles, and Gwen figures.