Mifune is in his prime. He can use anime feats.
Round 1: Weapons only.
Round 2: Jutsu allowed.
Round 3: All out.
Alright. Might as well make a case for Mifune since a few people think he is going to lose decisively here. Mobile at the moment so I can't post scans.
First, let me reiterate: this is prime Mifune who was at the age of 65 during the summit (Hiruzen was considered past his prime at 68/69 if I remember correctly.)
Unfortunately, due to lack of feats, Mifune's capabilities can be only measured based on statements, accolades and figts with Hanzo. First and foremost, he was stated to be too fast for ninja to make hand seals by both Hanzo and 4th databook. His style, called Iai, combined with stunning speed, exploited the opening created by seals to ensure his opponent cannot use any ninjutsu. This is the pinnacle of what a samurai strive to achieve. Large amount of samurais either began to use ninjutsu or outright became ninjas due to efficiency of such techniques. Mifune achieving the speed, skill and technique necessary to prevent ninjas of Hanzo caliber from forming seals is remarkable. The whole theme of Land of Iron is faith - discipline and belief strong enough to push you to the top of a mountain.
Mifune was seen fighting Edo-tensei Hanzo and defeating him decisively. He might have been rusty and way past his prime, but it was still someone hailed as the god of shinobi back on the day. Even during his youth, Hanzo stated that he couldn't afford to take off his mask against opponent of Mifune's caliber so as to avoid giving him a single opening (Mifune could have slice the poison sack in his side, paralyzing him.)
Hanzo's praise and the fight Mifune has given him back then are the two most important of his showings. It was the man who soloed the Sannins and was considered to be a legendary ninja after all.
Based on that alone, I don't think Sasuke stands even the slightest chance in round 1, with round 2 being debatable only because he might manage to pull off desperate amaterasu. Round 3 could really go either way. Incomplete Susanoo is a hard nut to crack. Mifune does have range attacks and chakra enhanced sword strikes (strong enough to send MS Sasuke backwards after blocking one) but I can see Sasuke pinning him at some point. Could very well exhaust himself. Mifune is just that good in my book. Plus he is patient and disciplined.
R1: Mifune. Iirc he beat Hanzo who fought off all three Sanin and was so strong he granted them that title in the first place for surviving against him. Mifune’s specialy is his sword, so he should win weapons only.
R2: Sasuke due to better feats and not versatility.
R3: Sasuke, same as above.
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