Fisher Tiger appears in 12 issues.
Aladdin is a brotula merman who is the doctor of the Sun Pirates.
Arlong is a sawshark fish-man. He was captain of the all fish-man crew, the Arlong Pirates, and a former member of the Sun Pirates. One of his enemies, Nami, was tricked into serving Arlong as a navigator to make maps of the entire planet so Arlong could conquer all of it. He is the primary antagonist of the Arlong Park Arc.
Choo is a smelt-whiting fishman and officer of the Arlong Pirates.
Gyaro is a pop eyed goldfish fish-man member of Macro Pirates. He was once a member of the Sun Pirates under Fisher Tiger.
Jimbei is a whale shark fish-man, who was once a member of the Seven Warlords. He is the helmsman of the Straw Hat Pirates. He was the second captain of the Sun Pirates after Fisher Tiger.
King Neptune is a coelacanth merman, and the king of the Ryugu Kingdom.
Koala was once a slave of the Celestial Dragons as a young girl but freed and returned home by Fisher Tiger. Now, she is a high ranking officer of the Revolutionary Army, and a Fish-Man Karate assistant instructor.
Kuroobi is a ray fish-man martial artist and officer of the Arlong's Pirates.
Macro is a macropharynx fishman and captain of the Macro Pirates.
Queen Otohime is a goldfish mermaid, queen of the Ryugu Kingdom, wife of King Neptune, and mother of Shirahoshi, Fukaboshi, Ryuboshi, and Mamboshi.
Tansui arowana fish-man member of Macro Pirates. He was once a member of the Sun Pirates under Fisher Tiger.
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