Draculina lived on Drakulon until she was cast out by her mother, Lilith, for plotting to over throw her and rule Drakulon as the new queen. Draculina somehow came to Earth and joined a government agency that monitors supernatural activities. After Vampirella prevented an apocalypse from taking place by Chaos and the Blood Red Queen of Hearts, Draculina had Vampirella, along with Adam Van Helsing and Pendragon kidnapped and taken to a facility after convincing her superiors that they were a threat to Earth.
Her real intentions were to use Adam, who still retained some of Chaos power to open a gateway portal to Hell, so that she could go back to Hell and become its ruler. In the stand-off, Vampirella was able to defeat Draculina by feeding on Adam's blood, which contained traces of Chaos blood, thus severing Draculina's control over Adam. In retaliation, Draculina opened the gateway dimension. The portal would have sucked Vampirella and her friends into the hell dimension, however, Vampirella along with Adam and Pendragon managed to destroy the machine in the nick of time.
Despite Vampirella wanting to help her little sister, Draculina threw herself in the portal, seemingly going back to Hell permanently.