The group was created sometime as early in 1909 by the German government in planning the start of World War I. Die Zwielichthelden is by considered to be more dangerous than their counterparts including the League and Les Hommes Mystérieux, which consists of their well organized leader Dr. Mabuse, mesmerist Dr. Caligari, his mind-slave Cesare, and Dr. Rotwang along with his female android Maria. Since its founding, the group's members had planned on furthering their personal agendas whereby each might profit from World War I and its aftermath.
When learning of the creation of Les Hommes Mystérieux and their knowledge of Germany's conspiracy, Die Zwielichthelden retaliated by manipulating their French counterparts into fighting the League into believing that their opposing group plot in creating World War I. Die Zwielichthelden's plans had worked with the help of Doctors Mabuse and Caligari into feeding British and French intelligence with false reports, allowing the German group's ambition to continue without interruptions.
The group was controlled by Adenoid Hynkel during the Second World War. Following the death of Carl Rotwang, the Berlin Metropolis was governed by Dr. Caligari and Maria.
Janni encountered the remnants of Die Zwielichthelden when she visited the Berlin Metropolis during 1941. Mabuse had grown unhappy with the rest of the group and assisted Janni and Jack in destroying them. Maria was destroyed and Caligari killed during that confrontation, and with Rotwang and Cesare having both died some time before; this left Mabuse as the sole surviving member of Die Zwielichthelden. He became an associate of Janni until his death, and is survived by his daughter Ursula.