Cassandra Cain appears in 547 issues.
Cassandra Cain infiltrated Aftermath's gang with some other members of the Justice League Elite, intending to take it down from the inside.
The mistress of a Roman themed assassin group.
The Bizarro World version of Supergirl. She is a twisted, doppelganger version of Supergirl. She is incredibly dangerous, scary, unpredictable and is wildly emotional, doesn't know her own strength, and is definitely a force to be reckoned with.
A ruthless Gotham mob boss and leader of the False Face Society known for his extreme hatred for Bruce Wayne and sadistic talent for torturing his victims.
Black Wind is a Meta criminal who upon first meeting Cassandra was instantly smitten by her.
Brutale was one of the super villains who attempted to kill Batgirl and Robin when they relocated from Gotham to Bludhaven.
Cricket is a deadly ten year old assassin who managed to beat both Black Bat and Red Robin.
One of the world's deadliest assassins, and the father of Cassandra Cain.
Longtime villain to the Titans and Teen Titans, Deathstroke took interest in Cassandra after they fought while he sought out Nightwing. Intrigued by her he later attacked her and had his own daughter Ravager face her in battle. When Cassandra mortally wounded her he vowed revenge.
When Batman left Harvey Dent in charge of Gotham and took Robin and Nightwing along for a trip around the world and to improve their skills Cassandra felt betrayed and abandoned Deathstroke managed to drug her during this time and made her leader of the League of Assassins and resulting in her commuting several murders.During this time he also made her a member of his Titans East, when Robin freed her she vowed to kill him for he had made her do but she was prevented from her goal by Nightwing.
Doctor Death was the very first supervillain Batman faced, way back during the Golden Age of comics. Typically portrayed as a disfigured mad scientist, he commonly operates as a creator of various biological weapons.
Douglas Haze was a serial killer known as the "Doll Man" after his paper doll trademark. He was captured by Batgirl.
An unrepentant Nazi Scientist, Dr. Dedalus is the mysterious agent of Leviathan, founder of Spyral, and the biological father of Kathy Kane, the original Batwoman as well as Spyral scientist Frau Netz. He terrorized Batman Inc but was killed by Damian Wayne to spare Batman
An ancient god who had been trapped within the Source Wall. She was later freed by Manchester Black who used her power to attack the Earth and the Justice League Elite.
A hired mercenary who clashed with batgirl early in her career
Gemini is the daughter of Madame Rouge, seeking revenge on her mother's killer, Beast Boy. She is a member of the Brotherhood of Evil, following in her mother's footsteps.
A group of cockroaches who gained sentience after being exposed to Joker chemicals and feeding on a pesticide scientist. Together gathered to fill the man's clothes and make a pseudo-human form. 'Kafka' went out into an alley where Spoiler saw 'him' eating a homeless man. Kafka fought off Batman and Spoiler and later fought Batman and Tarantula.
Kaliber is a gun-runner from Markovia. Batman is giving him a chance to redeem his past by working with the Outsiders.
New villain who believes Batman's proteges weaken him.
Lady Shiva is most likely the deadliest assassin in the DC Universe. Shiva has trained Batman and has also trained the third Robin, Tim Drake. Shiva's daughter, Cassandra Cain, would later become the second Batgirl.
As David cain's two children they came in conflict because he was a member of the league and was quite crazy and she wanted to save the lives of the people he was trying to kill.
The criminal father of Harper and Cullen Row, locked up in Blackgate.
An assassin whose is also one of David Cain's daughters, and half sister to Cassandra Cain. She wants to kill her father.
Mother of Harper Row and criminal murdered to make Harper undergo trauma
Gorilla apprentice and creation of the Brain whose IQ has been raised to the genius-level of 178.
Ultimate human trafficker seeking to remake the world using trauma to mold children into her image
After his wife became terminally ill, Dr. Victor Fries devoted his life to saving her and used cryogenics to freeze her to get time to find a cure. After being attacked by his boss Ferris Boyle and exposed to some of his chemicals, Fries could only survive in sub-zero temperatures and built a special suit to keep himself alive, becoming Mr Freeze.
Member of the Victim Syndicate and stockbroker fallen under the sway of Poison Ivy
Member of the Victim Syndicate and former owner of a joke store who had an allergic reaction to the Joker's toxin and required a tracheotomy
Originally created for the Batman animated series, Nora Fries was the cryogenically frozen wife of Mr. Freeze. His obsession to find a cure for her illness turned him to a life of crime. She also briefly became a supervillain named Lazara.
Nyssa took over the League of Assassins following her father Ras al Ghul's demise. She then makes a new League of Assassins and attempts to sway Cassandra into joining.
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