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Mary Jane Watson is an actress and a major love interest of Peter Parker aka Spider-Man. They have had a long and dramatic on-and-off-again relationship. She's one of the few people who know who he is and still supports him as a close friend and confidante.
Antón Miguel Rodríguez was a mercenary and assassin best known as the Tarántula; a foe to Spider-Man. Since his suicide, the Tarántula mantle has been passed on to other people.
A career criminal, the man known as Rhino has incredible strength and durability. A long-time enemy of Spider-Man and the Incredible Hulk, Rhino isn't particularly malicious, and has tried on various occasions to leave behind his life of crime, but always seems to slide back into old habits.
Mysterio was a formidable foe to Spider-Man. So much so that his arts of illusion were carried on by others after the original Mysterio's death. The original Mysterio, Quentin Beck, has recently returned. The events behind his resurrection are currently shrouded in mystery.
Swindled by his business partner out of his equal share of the profits from their company, Adrian Toomes set out for revenge by using a flying harness of his own design. He has however, met defeat several times at the hands of his arch-nemesis, The Amazing Spider-Man.
MacDonald "Mac" Gargan was a failing private investigator that was turned into the Scorpion with financial help from J. Jonah Jameson. He became a host of the Venom symbiote and was part of Norman Osborn's Dark Avengers. After the US Government took the symbiote, he became The Scorpion once again.
Frank Oliver with his superhuman leaping abilities was the criminal better known as the Kangaroo. After Frank's death, another person named Brian Hibbs took over as the Kangaroo.
Peter Parker was bitten by a radioactive spider as a teenager, granting him spider-like powers. After the death of his Uncle Ben, Peter learned that "with great power, comes great responsibility." Swearing to always protect the innocent from harm, Peter Parker became Spider-Man.
A former assistant to Curt Connors, Vincent Stegron used stegosaurus DNA to augment his body.
Dutch criminal with Cobra like powers, who went on as the third leader of The Serpent Society.
The world's first "live streaming super-villain." She commits crimes to get more hits on her webpage.
Tyrone Johnson is the "dark" half of the vigilante duo known as Cloak and Dagger. After a drug-related experiment awakened his powers, he became a living personification of darkness. Cloak is the Yin to his partner Dagger's Yang. Superheroes of the streets, Cloak and Dagger wage a war against dangerous drugs and drug-related crimes.
Tandy Bowen is the "light" half of the vigilante duo known as Cloak & Dagger. After a drug-related experiment awakened her powers, she became a living personification of light, with the ability to generate super-luminosity at will and use it both offensively and defensively.
Originally a replacement for the Human Torch in the 1978 Fantastic Four animated series. H.E.R.B.I.E. often provided comic relief with interactions with the Thing. He has since become an indispensable part of the Fantastic Four in the comics.
Teresa Parker is Peter Parker's long lost sister. She was on the run from the Kingpin when she revealed her connection to Peter Parker. She has recently returned to New York City.
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