While busting a gang of bank robbers, Spider-Man doesn't notice one of the criminals escape out a side window. That robber turns out to be the Chameleon, who has returned to the United States after facing deportation for his past exploits in crime. Seeing that Spider-Man is still a threat to his operations, the Chameleon decides to enlist the aid of an old friend - the world-renowned Kraven the Hunter - to take care of the web-slinger.
Hearing of Kraven's arrival, J. Jonah Jameson takes Peter Parker and Betty Brant to document the arrival of the big game hunter. After Kraven arrives on the docks, some of the wild animals that were also on-board escape. Parker springs into action, quickly changing into his Spider-Man costume, only to find that Kraven has single-handedly taken care of the beasts. Kraven then takes off to meet with the Chameleon.
Later that evening, Spider-Man takes care of some petty criminals, unaware that he is being watched from afar by Kraven. After dealing with the small-time hoods, Spider-Man runs across Kraven, and they have their first battle. During the fight, Kraven scratches the wall-crawler, injecting him with a potion that significantly weakens the web-head. Kraven then takes his leave, telling Spider-Man he wants to enjoy prolonging the web-slinger's defeat.
Feeling slightly better the next morning, Peter Parker learns that his Aunt May has arranged a blind date for Peter with the niece of next-door neighbor Anna Watson. To put more strain on Peter over the duration of his day, he seems to be developing a nasty case of the shakes caused by whatever potion Kraven used on him the prior evening. The trembles become so severe he can't even use his webbing accurately.
Deciding to search for Kraven, Spider-Man ventures out that evening, relying on his spider-sense to locate the big game hunter. Following him into a park, Spidey gets caught in a falling net. Freeing himself from the trap, Spider-Man finds himself surprised by what appears to be a second Kraven the Hunter. Confused by this sudden development, Spidey can't avoid Kraven's magnetic ankle and wrist cuffs. Hobbled by the device, Spidey sets out on the defensive while trying to free himself from the cuffs.
Succeeding in his attempt to nullify the cuffs' magnetic properties, Spider-Man then quickly unmasks the imposter Kraven to reveal the Chameleon. Following that revelation, Spidey chases the real Kraven through the park until he traps the hunter in an oversized web. Returning home, Peter finds that Anna Watson's niece had a headache and couldn't keep their blind date, which doesn't upset Peter all that much.